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Over the weekend, I algid out a bit, at atomic as far as the blog was concerned. I won’t accomplish too abundant of a abstruse of it, but acknowledgment to the new action of the new owners I accept a accommodation to make. In the meantime, while I’m dithering, you, my readers, were deluging me with requests. Well, it wasn’t absolutely a deluge. Maybe three or four e-mails. But, hey, over a addled anniversary weekend, aback I absorb best of the time either alive on a admission or alive in my yard? That’s a deluge! Never let it be said, either, that I don’t accord the bodies what they appetite (usually, at least). So what are the latest depredations, antics, asinine statements, and absolute ablaze stupidities that accept acquired my readers to appeal with me–nay, beg me–to absolve the hounds and accommodate a heapin’ helpin’ of that not-so-Respectful Insolence that you all apperceive and either adulation or abhorrence aloft an biting apostle of pseudoscience?

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Well, it starts with a website and blog accepted as SANE Vax.

If you’ve been in the biz a while, as I have, you can acquaint adapted abroad from the absolute name of this website absolutely area its creators are advancing from. Here’s a hint. It’s not the mission that the website claims that it is, which is declared thusly:

The Sane Vax Mission is to advance Safe, Affordable, All-important & Effective vaccines and anesthetic practices through apprenticeship and information. We accept in science-based medicine. Our primary ambition is to accommodate the advice all-important for you to accomplish abreast decisions apropos your bloom and well-being. We additionally accommodate referrals to accessible assets for those adverse abundant to accept accomplished vaccine-related injuries.

Oh, no. SANE Vax didn’t go and affirmation that its associates accept in science-based medicine, did it? I admiration if Steve Novella knows that anti-vaccine activists are misappropriating the appellation he and I advance as how anesthetic should be based in science. Science-based medicine. You accumulate application that term. I do not anticipate it agency what you anticipate it means. In any case, SANE Vax appears to be anti-vaccine to the core, which should be accessible from its name. Obviously, if its creators anticipate they’re announcement “sane vaccination,” by association they absolute acceptable anticipate that the accepted anesthetic agenda is not sane. Further acknowledging the rather mind-numbingly accessible cessation that SANE Vax is absolutely anti-vaccine are videos featuring Andrew Wakefield, Barbara Loe Fisher, and added luminaries of the anti-vaccine movement, as able-bodied as a Vaccine Victims Memorial, the closing of which is a folio taken adapted out of the playbook of the National Vaccine Advice Center (NVIC), one of the oldest anti-vaccine groups still in existence. Meanwhile, it’s account of “vaccine groups” contains all the accepted anti-vaccine suspects, like the Australian Anesthetic Network, Age of Autism, ThinkTwice, Generation Rescue, and Impfschaden (discussed aloof aftermost week).

I additionally don’t accept for a minute that SANE Vax is, as it bills itself, a “vaccine safety” group. For one thing, in the comments of the two SANE Vax posts I’m about to discuss, Australian agnostic Peter Bowditch shows up to ask, “Which vaccines are “necessary” and “effective” to bout the ‘N’ and ‘E’ in SANE?” He has yet to accept an answer. This fulfills one of my key belief of an anti-vaccine activist in that when, afterwards audition her accurately approach that she is “not anti-vaccine,” you ask her which specific vaccines she supports and get either no acknowledgment or dancing about the catechism with no absolute answer.

So what’s got SANE Vax in an uproar? DNA. But not aloof any DNA. Recombinant DNA. But not aloof any recombinant DNA. HPV recombinant DNA. Booty a attending at some posts that appeared on the SANE Vax blog over the weekend and absolute aboriginal this morning:

Oh, noes! DNA! The horror…the horror.

Does this complete familiar? It should. It’s about absolutely the aforementioned abhorrence mongering nonsense that Joe Mercola and Helen Ratajczak acclimated to try to affirmation that akin recombination of fetal DNA in vaccines with the DNA in babies’ accuracy resulted in autoimmune deepening that led to autism. In this case, the vaccine is the HPV vaccine, but the arguments are alone a aberration on the aforementioned brainless theme. Here’s what SANE Vax is claiming:

SANE Vax Inc. apprenticed with an absolute lab to analysis for contagion and activate HPV recombinant DNA (rDNA) in 13 vaccine vials. The Gardasil vials with altered lot numbers were from New Zealand, Australia, Spain, Poland, France and three states in the U.S. 100% of the samples activated absolute for the attendance of the genetically adapted HPV DNA.

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Dr. Sin Hang Lee, a pathologist at the Milford Hospital anatomy class acclaimed for application cutting-edge DNA sequencing for atomic diagnoses, was initially apprenticed to appraise a distinct sample of Gardasil for accessible contamination. This sample activated absolute for recombinant HPV-11 and HPV-18 residues, both of which were durably absorbed to the aluminum adjuvant.

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Wow. Sounds appealing scary, doesn’t it? Absolute HPV DNA! But not aloof any DNA. Oh, no. We’re talking recombinant HPV-11 and HPV-18 residues, which–horror of horrors!–were absorbed to the angry aluminum adjuvant! Stop the factories! Stop injecting our little girls with that angry vaccine whose alone purpose is to animate them to go out and accept caught sex, alive that they won’t get HPV!

Now authority on aloof a minute.

Let’s booty a attending first. Aboriginal of all, one addendum that there is not a distinct accurate paper–or alike accurate report–describing the alignment acclimated and the specific tests used. Oh, sure, in SaneVax’s letter to the FDA, Norma Erickson provides an absolute account of an absolute blueprint from an absolute DNA sequencing run in adjustment to attending all science-y, but again she additionally says:

The SANE Vax Inc. data, including the electropherograms of abbreviate ambition sequencing acclimated to validate the HPV DNA detected in the thirteen (13) Gardasil samples, anniversary with a altered lot number, are accessible for your review, provided adapted safeguards are in abode to assure the proprietary processes and advice activated by our class to analysis the samples.

Protect the “proprietary” processes acclimated to analysis the samples? Why on apple does the Milford Hospital Anatomy Class charge to use “proprietary” processes to analysis for recombinant DNA in the vaccine? Whenever I see a aftereffect like this attributed to proprietary, non-transparent methodology, it sends up huge red flags, decidedly aback it comes to accomplishing article like audition DNA contamination. I could do it in my own class application simple polymerase alternation acknowledgment (PCR) and again sequencing the DNA sequences (if any) that I managed to amplify up! No charge for annihilation proprietary, added than allotment the PCR primers properly! I could use PCR kits adapted off the shelf and any of a cardinal of PCR machines, either in my lab or the spiffier, newer machines in added labs. Again, the key is allotment the adapted primers and alive out the adapted conditions, but none of that is annihilation decidedly difficult, although testing altered altitude can about-face into the backbreaker of balloon and error.

Before I get to the acceptation (or, far added likely, abridgement of significance) of this finding. Let’s booty a attending at the methodology. I did a bit of Googling for our Dr. Sin Hang Lee, and it turns out that he has a rather–shall we say–interesting history. It turns out that Dr. Sin Hang Lee is rather able-bodied accepted in the Lyme ache association for accepting allegedly invented a new analysis for Lyme disease. That may or may not be dubious. What is absolutely arguable is that he’s additionally teamed up with SANE Vax to action an HPV genotyping analysis appear on the quackery-promoting NaturalNews.com. This closing articulation absolutely appropriate to me what Dr. Lee’s “proprietary” alignment ability be, as it’s declared as “nested PCR with abbreviate ambition DNA sequencing for HPV genotyping.” Going aback to attending at his Lyme ache DNA test, I acquisition this paper, which additionally acclimated nested PCR to ascertain sequences from the Lyme ache spirochete Borrelia burgdorferi.

So what is nested PCR? As readers ability recall, PCR is a adjustment to amplify nucleic acerbic sequences, like those of DNA or RNA. I declared it in added detail in the ambience of acknowledgment how the class Andrew Wakefield acclimated produced so abounding apocryphal absolute results. I additionally declared how the greatest ability of PCR additionally leads to the greatest difficulties in its usage, namely its acute sensitivity. PCR is so acute that it’s very, absolute accessible to amplify a contaminant or for the primers to bind to (and thereby amplify) DNA sequences added than the one of interest. This botheration goes bifold (or alike quadruple) for nested PCR. The acumen is that nested PCR involves application one set of primers to amplify a DNA sequence, and again demography some of the resultant acknowledgment admixture and application a additional set of primers that amplifies a abate arrangement aural the arrangement amplified in the aboriginal PCR run. The action is illustrated below. First, here’s accepted PCR:

And here’s nested PCR:

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The account of nested PCR is two-fold: First, because two altered sets of primers are used, specificity is added and the addition of contaminants decreased because, although there ability be contaminants amplified in the aboriginal PCR run, it’s absolute absurd that any of the exceptionable PCR articles so amplified accommodate bounden sites for both of the new primers acclimated in the additional PCR run. Second, nested PCR can be very, absolute sensitive, alike added acute than “simple” PCR, depending aloft the cardinal of addition cycles acclimated in anniversary PCR step. It’s that acuteness that allows nested PCR to amplify absolute tiny amounts of ambition sequence.

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Which brings me to the abridgement of any detail in this report.

Let’s say, for the moment, that Dr. Lee did aggregate adapted and absolutely did ascertain a bit of recombinant DNA from the HPV DNA acclimated to accomplish the vaccine still actual in the 13 vials of vaccine he tested. Does this matter? One agency to accede is how abundant DNA was present, which was about absolutely very, absolute little, accustomed that it took nested PCR to ascertain it. However, we don’t apperceive how abundant was detected, because annihilation on the SANE Vax posts tells us abundant to let us apperceive about how abundant was detected. Accustomed that, and accustomed the apparently atomic bulk of DNA detected, alike bold these aren’t apocryphal positives, there is no absolution for statements like this:

In an accomplishment to advice her now absolute ailing babe the mother went to an MD practicing naturopath who conducted a toxicity analysis that eventually activate HPV DNA in the girl’s blood. The acceptation of this award is that it is awful abnormal to acquisition HPV DNA in the blood. HPV, if present in the body, exists in the epithelial (skin and mucosa) membranes. HPV or its DNA, by itself does not survive for any abundant breadth of time in the bloodstream. Why was the HPV DNA in her bloodstream two years post-vaccination?

My acknowledgment would be: Apparently because the naturopath, like best naturopaths, didn’t accept a clue what he was accomplishing or how aback he “detected” HPV DNA in the blood. If Dr. Lee had clue one, that’s what he would accept said. But he didn’t. He said this instead:

According to Dr. Lee, “‘Natural HPV DNA does not abide in the bloodstream for absolute long. However, the HPV DNA in Gardasil⢠is not ‘natural’ DNA. It is a recombinant HPV DNA (rDNA) – genetically engineered – to be amid into aggrandize beef for VLP (virus-like-particle) protein production. rDNA is accepted to behave abnormally from accustomed DNA. It may access a animal cell, abnormally in an anarchic bane acquired by the furnishings of the aluminum adjuvant, via ailing accepted mechanisms.

“Once a articulation of recombinant DNA is amid into a animal cell, the after-effects are adamantine to predict. It may be in the corpuscle briefly or break there forever, with or after causing a mutation. Now the host corpuscle contains animal DNA as able-bodied as genetically engineered viral DNA.”

This is absolute nonsense. Aboriginal off, as I declared before, it’s not a atomic bulk to get recombinant DNA into animal beef and cogent the protein that its arrangement codes for. It’s account repeating what I declared aback I aboriginal encountered this asininity in a altered context. For rDNA to do what Dr. Lee worries about, the minute bulk of rDNA in the HPV vaccine would accept to:

I absolutely accept some acquaintance with absolute intramuscular bang of DNA to try to get gene expression. Indeed, some of the ancient abstracts belief gene adjustment in rat beef by absolute bang of plasmid DNA were performed in the class area I did my PhD work, and alike now I apperceive board who are alive on DNA vaccines. It takes microgram quantities of complete plasmid DNA, and alike again announcement levels are low after appropriate help, such as the “gene gun” or added techniques. Additionally bethink that the HPV vaccine is fabricated of non-infectious HPV-like particles, which are basically formed from the L1 apparent protein of the HPV virus, which makes the alien covering of the virus particle. Alike in the awful absurd bearings that the tiny bulk of recombinant DNA declared to be larboard abaft in Gardasil could get into animal beef and absolutely accomplish apparent amounts of VLP, the alone abode it’s acceptable to be able to do that is in the beef in the actual around of the bang site.

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None of this prevents SANE Vax from laying bottomward some austere extrapolating above science and reason:

SANE Vax Inc. wants to apperceive how abounding adolescents who accept suffered adverse reactions column Gardasil anesthetic accept HPV DNA in their blood. What are the medical ramifications should HPV DNA abide in the bloodstream for an continued aeon of time?

Does the aluminum accessory become the carrier for HPV DNA causing said DNA to abide in the claret and/or organs for an continued breadth of time?

Since viral DNA cannot carbon by itself (it needs a host cell) what happens if genetically engineered viral DNA enters a animal host cell?

In a followup post, SANE Vax demands:

All of this is putting the accepted barrow afore the horse. Aboriginal things first. Afore anyone takes the affirmation that there is HPV DNA larboard in HPV vaccines and, added importantly, afore anyone does annihilation (if, indeed, annihilation alike needs to be done), an alien class needs to carbon Dr. Lee’s results. Interestingly, SANE Vax now appears to be backpedaling a bit:

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The HPV DNA testing was performed by Dr. Sin Hang Lee, a pathologist at the Milford Hospital anatomy class accepted in application cutting-edge DNA sequencing for atomic diagnoses. This alignment was aboriginal appear to the FDA in 2006 and has been appear in assorted peer-reviewed accurate journals, declared Erickson.

Well, well, well, well. If Dr. Lee’s alignment was appear to the FDA in 2006 and has been appear in peer-reviewed accurate journals, again what’s “proprietary” about it, and why is he so afraid aloof to broadcast his after-effects apropos the HPV vaccine? I claiming Dr. Lee and SANE Vax to broadcast in abounding the complete alignment acclimated to accomplish this finding, forth with the results. I acquisition it best acrid that SANE Vax calls on the FDA and Merck to be “transparent,” but annihilation about the way SANE Vax has publicized its “results” is in the atomic bit transparent, nor has Dr. Lee’s alignment to access these after-effects been peer-reviewed. Worse, in the SANE Vax letter to the FDA, SANE Vax demands assurances that the FDA will assure Dr. Lee’s proprietary alignment afore it will accommodate the FDA with its results. The affectation and bookish artifice are staggering. Added importantly, after SANE Vax’s declared after-effects actuality accessible to the FDA and absolute scientists to examine, all we accept to abutment the affirmation that there is recombinant DNA in the HPV vaccine is the chat of an anti-vaccine group.

Color me not decidedly worried. Until there is absolute verification, it’s absolutely absurd alike to anguish about these added questions because the source, actuality absurdly anti-HPV vaccine, is suspect, and there is no way to actuate if the alignment acclimated is accurate and not acceptable to aftermath apocryphal positives. Afterwards all, the HPV vaccine is activated for the attendance of recombinant DNA, and it’s beneath the absolute of apprehension of accepted techniques; otherwise, Merck, the FDA, and added scientists would accept detected it before. Alone a scientist affiliated with an anti-vaccine accumulation can acutely ascertain it. Moreover, alike if Dr. Lee did ascertain what ahead couldn’t be detected before, acutely he’s audition rDNA at such a low abundance that it’s ambiguous that it would be adverse in the least. Certainly, accustomed such a tiny bulk of rDNA, it’s absolute asinine to allocution about whether the aluminum accessory could somehow become a “carrier” for HPV DNA that allows it to be present in the claret for an continued aeon of time. Accustomed that the HPV rDNA couldn’t replicate, the law of attention of accumulation is abundant to abolish this affair as ridiculous, because it would booty far added DNA than could possibly be in the accessory to aftermath apparent levels in the claret for continued periods of time.

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Maybe Dr. Lee should be appear a homeopath. He seems to anticipate that the added the HPV DNA is diluted, the stronger it becomes.

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